[Clinical and morphological characteristics of Ewing's sarcoma and the algorithm for diagnosing undifferentiated round cell sarcomas]. / Kliniko-morfologicheskaya kharakteristika sarkomy Yuinga i algoritm diagnostiki nedifferentsirovannykh kruglokletochnykh sarkom.

BACKGROUND: The group of undifferentiated round cell sarcomas, according to the World Health Organization Classification, in addition to Ewing's sarcoma (ES), includes round cell sarcoma with rearrangement of the EWSR1 gene with partners not from the ETS gene family, sarcoma with BCOR gene alterations, CIC -rearranged sarcoma. Despite the fact that all tumors have clear histological and immunological criteria, their diagnosis can be difficult, given the fact that there are overlapping variants of the morphological picture and immunophenotype both within the group and with other round cell tumors. OBJECTIVE: Present a comparative analysis of genetically verified ES, sarcoma with BCOR gene alterations and CIC-rearranged sarcoma. MATERIAL AND METHODS: A comparative study of biopsy specimens of bones, soft tissues and internal organs was carried out in 118 patients with ES, 10 with BCOR gene alterations and 8 with CIC-rearranged sarcomas. All cases were genetically verified. The following research methods were used: histological, immunohistochemical, RT-PCR, RNA sequencing and FISH. RESULTS: Within our cohort, it was shown that ES predominantly affects bones, while soft tissue localization is more typical for the other two undifferentiated round cell sarcomas. Histologically, in the overwhelming majority of cases, ES is characterized by a monomorphic round-cell structure; on the contrary, heterogeneous structure is typical for sarcoma with alterations of the BCOR gene, CIC-rearranged sarcoma. High sensitivity and specificity of CD99/NKX2.2 co-expression for ES, BCOR/SATB2/TLE1 for sarcoma with BCOR gene alterations, high specificity and low sensitivity of WT1/ETV4 co-expression for CIC-rearranged sarcoma was shown. CONCLUSION: For the differential diagnosis of undifferentiated round-cell sarcomas, it is necessary to take into account the clinical, morphology when compared with the data of the IHC study, and verification by molecular genetic methods is necessary to improve the accuracy of diagnosis.

[CIC-rearranged sarcoma: a case report and literature review]. / CIC-perestroennaya sarkoma: klinicheskoe nablyudenie i obzor literatury.

CIC-rearranged sarcoma is a rare and extremely aggressive tumor that occurs mainly in soft tissues. Despite the fact that identification of a characteristic genetic rearrangement is necessary to verify the diagnosis, in most cases, the correct diagnosis can be made by comparing histological signs and a characteristic immunophenotype, which greatly speeds up the diagnosis. The article describes a case of CIC-rearranged sarcoma in a 14-year girl with the successful application of the CWS-2009 treatment protocol.

[Angiomatoid fibrous histiocytoma: a literature review and a report of two cases]. / Angiomatoidnaya fibroznaya gistiotsitoma: obzor literatury i opisanie dvukh nablyudenii.

Angiomatoid fibrous histiocytoma (AFH) is a rare soft tissue tumor of intermediate malignancy potential, which predominantly affects children and young adults. This paper describes two cases of AFH, as well as a review of literature during 1979 to 2021. It gives data on the epidemiology, clinical features, diagnosis, and genetic characteristics of AFH.

[Three-dimensional scanning of the objects of forensic medical expertise]. / Trekhmernoe skanirovanie sudebno-meditsinskikh ob''ektov: osobennosti i perspektivy ispol'zovaniia v podrazdeleniiakh biuro sudebno-meditsinskoi ékspertizy.

The objective of the present study was the evaluation of the possibility for the application of the technology of universal three-dimensional scanning of the objects of forensic medical expertise in the practical work. The article reports the results of a pilot and an experimental studies designed to elucidate the potential of the equipment for the formation of the visualization algorithms under the different illumination and calibration conditions. The prospects for the recording and analysis of the three-dimensional images for the purpose of practical forensic medical expertise are discussed.

[Three-dimensional scanning of the forensic medical objects: the instrumental support and peculiarities of the technology]. / Trekhmernoe skanirovanie sudebno-meditsinskikh ob''ektov: pribornoe obespechenie i osobennosti tekhnologii.

The objective of the present study was to develop 3D-technologies for the investigations of objects for to purpose of their forensic medical expertise and to propose the rationale for their practical application. The advantages and disadvantages of the commercial 3D-scanners are disused. The development of a new original 3D-scanner is described. The data on the authors' technology for three-dimensional scanning of the forensic medical objects are presented.

[Organ-sparing surgery for nephroblastoma in children].

We present the results of 53 stage I-III nephroblastoma pediatric patients treated in Belarusia from 2005 to 2010. All the patients received adjuvant chemotherapy (according to SIOP WT 2001 protocol), surgery (complete or partial nephrectomy) and neoadjuvant chemotherapy. The prolonged adjuvant chemotherapy was proposed in patients with good response as a cytoreductive measure enabling organ-sparing surgery. Five-year event-free survival was 91+/-6% in complete and 89+/-9% in partial nephrectomy recipients (p=0,64). Our experience suggest the feasibility of prolonged adjuvant chemoradiotherapy in patients with good clinical response and no contraindications for complete nephrectomy.

[Age-related changes of exercise capacity and some biochemical indices of rat muscles under influence of different light conditions and pineal preparations].

The influence of different light conditions (standard--12 h light : 12 h darkness-- LD; 24-hour constant light--LL, light deprivation--DD, natural light regimen of the North-West of Russia--NL) and substances with geroprotective effect (melatonin and epitalon) on age-related dynamics of exercise capacity, lactate dehydrogenase (LDH) isoenzymes spectrum, enzymatic and non-enzymatic system of generation and utilization of oxygen reactive substances, activity of key antioxidant enzymes in skeletal musculature were investigated during 2 years. The decrease of exercise capacity, changes of energy production strategies and antioxidant protection during animals ageing in dependence on different light conditions was found. LL lead to earlier decrease of exercise capacity with synchronous increasing content of less effective anaerobic LDH fraction and decreasing of catalase activity in comparison with DL. Similar decreasing of exercise capacity, changes in LDH spectrum and antioxidant status in NL condition coincide in time with autumn season what indicates that seasonal changes of illumination is natural disturber of circadian rhythm. Melatonin and epitalon applied from 4-month age did not influence on exercise capacity in young rats. Both substances had similar stimulate effect on age-related physical activity of mature and senescent animals such as reducing of exercise capacity depression and normalization of antioxidant protection.